In Lap Lambert Academic Publishing In Vitro Plant Propagation and Conservation by Tissue Culture Paperback

This book presents an efficient in vitro propagation protocol for Gloriosa superba Linn., an endangered medicinal plant, and Plumbago indica L., a rare medicinal plant. Optimized culture conditions successfully promoted shoot induction and multiplication in both species. For G. superba, MS medium supplemented with 4.0 mg/L BAP and 1.0 mg/L IAA produced shoots in 92% of cultures, with an average of four shoots per culture. For P. indica, MS medium containing 0.5 mg/L BAP resulted in shoot production in 95% of cultures, with six shoots per culture. The addition of casein hydrolysate, activated charcoal, and coconut water further enhanced shoot multiplication, producing up to 12 and 14 shoots per culture, respectively. Species-specific auxin treatments also promoted successful rooting of in vitro raised shoots. The protocol offers a practical and promising approach for the rapid propagation, sustainable utilization, and conservation of these valuable medicinal plants.